Cells are the basic units of life. They determine normal organ function and disease development. Observations of living cells are vital to understanding, diagnosing, and successfully treating disease. For the past decades, invasive procedures and microscopic analyses have been used to evaluate changes in cellular morphology and distribution in response to pathologic conditions and associated treatments. Recent technological advances have enabled us to develop non-invasive imaging techniques that can generate detailed information about specific cells in the body. Over the past years, our research team translated several cellular imaging technologies from preclinical research concepts to clinical applications, thereby creating direct value for our patients.